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Improvement of Fermentation of Dried Distillers’ Grains and Solubles (DDGS) Hydrolysates to Acetone Butanol and Ethanol (ABE) with Hydrolysate-adapted Clostridium beijerinckii BA 101

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Bioenergy Engineering, 11-14 October 2009, Bellevue, Washington  BIO-097951.(doi:10.13031/2013.28867)
Authors:   Yi Wang, Xiaojuan Wang, Thaddeus Ezeji, Hao Feng, Hans P Blaschek
Keywords:   Acetone butanol ethanol (ABE), Adaption, Clostridium beijerinckii BA 101, DDGS, Inhibition, Tolerance

Formation of compounds that are toxic to fermenting organisms during pretreatment and hydrolysis of lignocellulosic biomass has long been identified as one of the key factors affecting bioconversion of biomass to biofuels. In this study, fermentation was carried out to convert electrolyzed water pretreated dried distillers grains and solubles (DDGS) hydrolysates to acetone butanol ethanol (ABE) using the hyper-butanol producing Clostridium beijerinckii BA 101. The presence of inhibitors in the DDGS hydrolysates resulted in a 40 h adjustment phase before the initiation of fermentation while the mixed sugar control fermentation was completed in less than 16 hours. While the ABE yield (ABE produced per unit of sugar utilized) obtained from the fermentation of DDGS hydrolysates was comparable to the yield obtained from the mixed sugar control fermentation, ABE productivity (ABE produced per unit of fermentation time) from the DDGS hydrolysates was lower than that of the mixed sugar control due to the long adjustment time experienced by C. beijerinckii BA101 in DGGS hydrolysates fermentation.

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