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Presence of excessive amount of authorized iron and manganese in water resources, always create different problems. Different methods have been developed for removal of iron and manganese. Of this achievement we used rapid sand filters. This kind of filters is extensively used for water and wastewater treatment. In this study different concentration of iron and manganese in water presence of different concentration of sulfate and in different rate of filtration are passed through filter and then the effluent is examined for present concentration of the iron and manganese, then the efficiency of the filter is evaluated for each step in this approach. The efficiency of manganese removal was lower than iron removal efficiency. The data was analyzed by creating a factorial pattern and using the analyzed variance table. The result shows that the effects of filtration rate, iron and manganese concentration in the influent and different concentration sulfate on the efficiency of the rapid sand filter for removal of iron and manganese was 99% significant.