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Pythium aphanidermatum is the largest remaining hurdle in the development of commercial hydroponic spinach production. To control this pathogen nutrient solution treatment technologies need to be selected. To aid in this selection process a Pythium bioassay utilizing 48 hour old spinach seedlings was developed. Damage from Pythium zoospores can be categorized as lesions, external discolorations, light internal streaking, and dark internal streaking, representing increasing seriousness of damage. These categories can be measured and combined into an overall root damage rating which allows comparisons between the different infecting solutions.