Click on “Download PDF” for the PDF version or on the title for the HTML version.
If you are not an ASABE member or if your employer has not arranged for access to the full-text, Click here for options.
Nucleic Acid Sensor with Fluid Handling Components for the Detection of Salmonella enterica
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.orgCitation: 2007 ASAE Annual Meeting 077103.(doi:10.13031/2013.23443)
Authors: Andrew T Csordas, Michael J Delwiche, Jeri D Barak
Keywords: real-time PCR, SYBR Green, pathogen detection, embedded controller, automated system, food safety
An embedded controller with a keypad user interface was used to operate a nucleic acid sensor with fluid handling and real-time polymerase chain reaction (PCR) capabilities. The sensor system consisted of pumps and valves for fluid transfer, a fluorometer composed of an LED, photodiode and signal conditioning circuitry, a thermoelectric module (TEM) for heating and cooling the reaction chamber, and power switching circuitry. The amplification and dissociation results of the SYBR Green real-time PCR test can be downloaded to a PC for evaluation.(Download PDF) (Export to EndNotes)