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Nucleic Acid Sensor with Fluid Handling Components for the Detection of Salmonella enterica

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  2007 ASAE Annual Meeting  077103.(doi:10.13031/2013.23443)
Authors:   Andrew T Csordas, Michael J Delwiche, Jeri D Barak
Keywords:   real-time PCR, SYBR Green, pathogen detection, embedded controller, automated system, food safety

An embedded controller with a keypad user interface was used to operate a nucleic acid sensor with fluid handling and real-time polymerase chain reaction (PCR) capabilities. The sensor system consisted of pumps and valves for fluid transfer, a fluorometer composed of an LED, photodiode and signal conditioning circuitry, a thermoelectric module (TEM) for heating and cooling the reaction chamber, and power switching circuitry. The amplification and dissociation results of the SYBR Green real-time PCR test can be downloaded to a PC for evaluation.

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