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Baled Canadian timothy hay underwent testing at a hay processing plant in order to determine if compression could be used as a disinfestation protocol for Hessian fly (Mayetiola destructor (Say)) puparia. This has been the second field testing conducted for this project. Pressure sensitive films were randomly distributed; throughout the material, on the chamber walls, and in different orientations, to assess the hay rebaler units ability to induce uniform pressure. Attached to the pressure films were organdy cages containing wheat seedlings infested with Hessian fly puparia. The variables which were tested included the hold time (0.5 and 2.0 s), applied pressure (10.34 and 12.41 MPa), and timothy hay quality (low moisture first cut, high moisture first cut and high moisture second cut hay). A total of 36 tests were completed which included 3 replicates of each condition. Within each test, 10 Hessian fly cages and 10 pressure sensitive films were used. Each fly cage contained approximately 168.56 Hessian puparia, translating into a total Hessian fly count of 60681 for the entire field test. Analysis of the pressure sensitive films showed that 100% of the hay experienced at least 200 kPa (29 psi) of pressure. Following the 75 day post experiment emergence period 5 (0.008%) of the puparia survived from one of the cages in test 4 and one cage in test 5.