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A Real-time Biosensor for the Detection of Pathogens

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  057027,  2005 ASAE Annual Meeting . (doi: 10.13031/2013.19930) @2005
Authors:   Andrew T. Csordas, Michael J. Delwiche, Jeri Barak
Keywords:   Salmonella enterica, SYBR Green, dissociation analysis, filtration mechanism

A real-time PCR system was developed and tested using a glass capillary tube and fiber optic cables for light transmission. The system was tested with extracted Salmonella enterica Newport DNA at concentrations ranging from 1000 to 1 ng per 50 l reaction. Dissociation peaks were detected at the target fragments melting temperature of 81C in all cases of extracted DNA samples tested. A filtration system for capturing bacteria from 100 ml samples of water was tested. Experiments with non-pathogenic E. coli O137:H41in sprout irrigation water indicated that it was possible to increase the cell density of the 100 ml sample by 6.6 times by back-flushing bacteria from a 0.45 m filter into 10 ml of water.

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