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Click on “Download PDF” for the PDF version or on the title for the HTML version. If you are not an ASABE member or if your employer has not arranged for access to the full-text, Click here for options. Monitoring Starch Saccharification Using Low Field NMRPublished by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org Citation: Paper number 057018, 2005 ASAE Annual Meeting . (doi: 10.13031/2013.19656) @2005Authors: Fu, R., Qi, J., Ruan, R. and Chen, Xiangyang Lin, Xiaofei Ye, Chris Doona and Tom Yang Keywords: Nuclear magnetic resonance, NMR, relaxation, ethanol, fermentation, saccharification, glucose The present study is an attempt to develop a low field NMR based technique for fast analysis of enzymatic saccharification of corn starch. Experiments were first conducted on model samples containing pure starch and glucose to prove the hypothesis. Starch saccharification was carried out using two commercial enzymes, and monitored using CPMG NMR pulse sequence using a low field NMR system (0.5 T, RI Maran DRX NMR Imaging System). NMR-determined and conventional method-determined glucose concentrations were compared. Data obtained using the CPMG pulse sequence were fitted to a multi-component model. Good linear correlations between NMR relaxation parameters (relaxation time and amplitude) and concentrations of starch and glucose were observed. (Download PDF) (Export to EndNotes)
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