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This study aimed at a preliminary evaluation of the feasibility of producing biodiesel using oil extracted from healthy and defective coffee beans. Direct transesterification of triglycerides from refined soybean oil and from oils extracted from healthy and defective (PVA) coffee beans was performed. There was good phase separation after reactions using oils from both soybean and healthy coffee beans. This was not observed when using PVA oil, probably due to saponification of free fatty acids during reaction. The highest conversion value (~90%) was attained for transesterification of oil from healthy coffee beans at 55oC during 1 hour. An increase in reaction temperature resulted in an increase in biodiesel conversion. Lower conversion values were obtained when using PVA oil, as a consequence of the presence of saponified free fatty acids, which hindered an effective contact among reagents.