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Fluorescence and hyperspectral reflectance scattering have recently been researched for measuring fruit post-harvest quality and condition and they are promising for nondestructive detection of fruit quality. The objective of this research was to use hyperspectral reflectance and fluorescence for measuring apple fruit quality. A blue laser (408 .) and a quartz tungsten halogen light were used as light sources for generating laser induced fluorescence and reflectance scattering in apples, respectively. The laser induced fluorescence and reflectance of Golden Delicious apples were measured by using a hyperspectral imaging system. Fruit firmness and soluble solids and acid content were measured using standard destructive methods. Principal component analyses were performed to extract critical information from both hyperspectral reflectance and fluorescence data and this information was then related to fruit quality indexes. The fluorescence models had poorer predictions of the three quality indexes than the reflectance models. However, the prediction models of integrating fluorescence and reflectance performed consistently better than the individual models of either reflectance or fluorescence. The correlation coefficient for fruit firmness from the integrated model was 0.86 and the standard error was 6.97 N.