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The objective of this study was to examine the temporal variation of greenhouse gas (GHG) concentration in the swine building over both daily and seasonal basis. The air samples were collected every one hour continuously for three days during summer and spring, and analyzed by gas chromatography (GC). Barn temperature was collected and the management practices were also noted. Results showed that methane (CH4) and carbon dioxide (CO2) concentration was related to the internal temperature and ventilation. Daily CH4 and CO2 concentrations varied more during cold weather than warmer weather; nighttime GHG concentration in the gestation building was higher than daytime because of the low air exchange. Average CH4 concentration in the gestation building was 16.67 + 9.88 ppm in spring and 9.25 + 7.64 ppm in summer. Average CO2 concentrations were 2361.65 + 960.96 ppm in spring and 1134.96 + 373.53 ppm in summer.