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Concerns over odor and gas emissions from livestock production facilities have increased in the United States. Biofilters have been demonstrated to be an effective technology in reducing odor and gas emissions from these facilities. Large biofilter media beds sometimes prevent application of this technology to building layouts. A vertical biofilter may be an effective alternative. The research objective was to determine optimum biofilter construction to achieve uniform airflow as the media settles. Media thickness was determined to be a factor in achieving uniform airflow. For a minimum thick biofilter media of 30 cm, a parallel wall construction produced the least airflow variation. As the media thickness increased to 61 cm, airflow variation across the biofilter was reduced by increasing the taper between the side walls which support the media to 9.6 degrees. Improved media moisture distribution was achieved when the water soaker hose was placed on top of the media versus being suspended vertically through the media. The net value of this research will achieve a more efficient vertical biofilter.