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Use of electrolyzed oxidizing water to inactivate Escherichia coli O157:H7 and Listeria monocytogenes Scott A on raw salmon

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  046020,  2004 ASAE Annual Meeting . (doi: 10.13031/2013.16925) @2004
Authors:   Nil P. Ozer, Ali Demirci

Raw fish is prone to risk of microbial outbreaks due to contamination of pathogenic microorganisms. Escherichia coli O157:H7 and Listeria monocytogenes are among the pathogens associated with raw fish. Therefore, it is important to treat raw fish to inactivate pathogenic microorganisms. Electrolyzed oxidizing water is novel antimicrobial agent containing acidic solution with a pH of 2.6- 2.9, ORP of 1120 – 1180 mV, and 76-90 ppm free chlorine, and alkaline solution with a pH of 11.5 and ORP of – 795 mV. This study was undertaken to evaluate the efficacy of electrolyzed oxidizing (EO) water for inactivation of E. coli O157:H7 and L. monocytogenes Scott A on the surfaces (muscle and skin surfaces) of inoculated salmon fillets. Inoculated salmon fillets were treated only with acidic EO water at 22°C and 35°C and sodium hypochlorite solution (90 ppm free chlorine) as control at 22°C for 2, 4, 8, 16, 32, and 64 min, respectively. For the treatment with alkaline EO water followed by acidic EO water, a response surface model was developed to predict effective times in the range of 5-30 min and temperatures in the range of 22-35°C for both alkaline and acidic water treatments. The acidic EO water treatments resulted in reductions of population of L. monocytogenes Scott A ranging from 0.40 log10 CFU/g (60 %) at 22oC to 1.12 log10 CFU/g (92.3 %) at 35oC. Treatment of inoculated salmon fillets in acidic EO water reduced E. coli O157:H7 populations by 0.49 log10 CFU/g (67 %) 22°C and 1.07 log10 CFU/g (91.1 %) at 35°C, respectively. Response surface analysis for alkaline EO water treatment followed by acidic treatment demonstrated that, maximum log reduction of 1.33 log10 CFU/g (95.3 %) for E. coli O157:H7 and 1.09 log10 CFU/g (91.9 %) for L. monocytogenes Scott A.

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