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Evaluation challenges in NIOSH-funded agricultural health and safety centers include those related to the current state of development of agricultural health and safety as a public health arena, those inherent in multi-site evaluation of diverse centers, and those related to evaluation itself. Challenges posed by the current state of development of the agricultural health and safety field include lack of established markers and measures for health and safety problems. The multi-site nature of centers, the tasks of which include interdisciplinary research, education, and intervention, is another source of difficulties. Challenges related to evaluation itself include lack of evaluation resources, both human and financial. This article makes three suggestions for advancing evaluation in agricultural health and safety centers: the adaptation of evaluation to the agricultural health and safety arena; evaluation capacity-building in the centers; and the development of national evaluation standards.