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Response Surface Modeling for Cleaning and Disinfecting Materials Used in Milking Systems with Electrolyzed Oxidizing Water

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  Paper number  037018,  2003 ASAE Annual Meeting . (doi: 10.13031/2013.15342)
Authors:   Stephen P. Walker, Ali Demirci, Robert E. Graves, Stephen B. Spencer, Robert F. Roberts
Keywords:   Milking systems, electrolyzed oxidizing water, CIP, clean in place, ATP bioluminescence

Electrolyzed oxidizing (EO) water is a technology that electrolyzes a weak sodium chloride solution into sodium and chlorine, resulting in two solutions alkaline and acid. The goal of this research was to determine if EO water is an acceptable cleaning agent for pipeline milking systems. Small pieces of materials commonly used milking system were soiled using raw milk inoculated with a cocktail of four bacterial cultures commonly found in raw milk, and then cleaned by soaking in the alkaline EO water followed by a soaking in the acidic EO water at various treatment times and temperatures. Effective time/temperature combinations were determined by response surface design for a temperature range of 25-60oC, and time range of 5-20 min. Aerobic plate counts and ATP bioluminescence were used to asses the cleaning and disinfecting. Several treatment conditions successfully removed all detectable bacteria and ATP, which demonstrated the effectiveness of EO water. Regression analysis of the ATP data resulted in a response surface model that shows all treatment parameters are significant factors. Response surface model can be useful tool in selecting treatment parameters for large scale experiments.

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