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A Conductometric Biosensor for Bovine Viral Diarrhea Virus Detection

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Paper number  037007,  2003 ASAE Annual Meeting . (doi: 10.13031/2013.14170) @2003
Authors:   Zarini Muhammad Tahir, Evangelyn C. Alocilja, Daniel L. Grooms
Keywords:   Polyaniline Bovine Viral Diarrhea Virus, conductometric, biosensor, polyaniline

Bovine viral diarrhea virus (BVDV) is one of the most insidious and economically devastating viral pathogens of cattle. Due to the complex pathogenesis and insidious nature of BVDV infections, a rapid and accurate diagnosis of BVDV becomes an important component to control and prevent the infections. Recently, a conductometric biosensor has been developed for its detection. The conductometric design is based primarily upon the specific nature of labeled antibody-antigen binding. The architecture of the biosensor demonstrates the advantages of using lateral flow format strip attached to a portable circuitry for signal measurement. Results show that the biosensor can detect a very low concentration of virus in less than 6 minutes. Details on the biosensor performance for BVDV detection in the enrichment media are presented. With the development of the conductometric biosensor for BVDV detection, further adaptation could be made for detecting other pathogens important in the livestock industry.

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