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Adsorption Kinetics of Cryptosporidium parvum to Soils

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org

Citation:  Paper number  034127,  2003 ASAE Annual Meeting . (doi: 10.13031/2013.13882) @2003
Authors:   S. J. McLaughlin, P. K. Kalita, R. A. Cooke, S. P. Fox, M. S. Kuhlenschmidt
Keywords:   Vegetative Filter Strip, Water Quality, Animal Waste Reduction, Adsorption

Vegetative Filter Strips (VFS) have long been used to control the movement of pollutants, thus preventing them from reaching receiving waters. Previous studies have shown a reduction in pathogen transport through a VFS, but little is known about the reduction mechanisms. In this study, Cryptosporidium parvum (C. parvum) has been investigated for adsorption properties with individual soil particles: sand, silt and clay. Clay and silt were incubated individually as well as with C. parvum oocysts and counted in a Coulter Counter. Sampling times started at zero time and were taken at various intervals ending at 210 minutes. The sand was too large to fit through the 100 m aperture, so results were generated using fluorescent and differential interference contrast microscopy. Photomicrographs were also taken of the combined incubations of oocysts with clay and silt to validate results from the Coulter Counter. Our results indicate that oocysts primarily adhere to or become entrapped within aggregating clay particles and do not adhere substantially to either silt or sand.

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