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Optical Techniques for Assessing the Fruit Maturity Stage
Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan www.asabe.org
Citation: Paper number 026041, 2002 ASAE Annual Meeting . (doi: 10.13031/2013.10585) @2002
Authors: Luigi Bodria, Marco Fiala, Riccardo Guidetti, Roberto Oberti
Keywords: Fruit quality, Chlorophyll content, Chlorophyll fluorescence, Nondestructive measurements, Red pigmented fruit
Among the several changes that a fruit undergoes during ripening, chlorophyll
degradation is responsible for degreening of ground color, that is a well established maturity indicator
for several species. In red pigmented cultivars of apples and peaches, of high interest for the
European market, this process is not visible, being masked by a uniform layer of anthocyanins.
Two different systems were developed to evaluate non-destructively the chlorophyll content in these
fruits, basing on their optical properties, in order to assess their maturity stage allowing for an
optimal, quality oriented harvest and post-harvest management.
A fluorescence imaging system equipped with a blue actinic light, allowed to obtain fruit's
fluorescence images in which the gray level of pixels resulted well correlated with firmness of fresh
apples (R2=0.81) and permitted to follow the post-harvest evolution of firmness and sugar content of
stored apples, even in absence of significant skin colour changes. The system equipped with a red
actinic light provided results fairly good correlated with firmness of fresh peaches and nectarines,
even though the fluorescence signal resulted quite low due to the low chlorophyll content in the
considered cultivars.
A dual-band, laser-diode based, punctual reflectance probe was developed and tested on fresh
peaches and stored apples. The index R/IR, defined as the ratio of the signal measured in red and
near-infrared band, was found to correlate the chlorophyll content of the fruits with R2=0.66,
regardless the differences due to the species. Moreover, R/IR resulted a fairly good estimator of
conventional quality indices (correlations R2=0.4-0.5 with sugars and firmness), and allowed to track
the post-harvest ripening process, showing different evolution patterns for fresh peaches at different
maturity stages.
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