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Outbreaks of bacterial infection with agents such as L. monocytogenes, Salmonella spp., M. paratuberculosis and Escherichia coli O157:H7 have been linked with cattle manure. Indicator organisms have been employed traditionally for assessing fecal contamination and treatment system evaluation. In this study, environmental temperature and manure moisture content impact on indicator (fecal coliforms, E. coli and fecal streptococci) survival and release were investigated, a new approach was assessed to obtain the maximum number of indicator bacteria release into water. The effects of three temperature (4, 27 and 41 C) and three moisture content (30%, 55% and 83%) levels on bacterial populations were studied. Temperature was found to have a significant overall effect whereas moisture content of the manure was not. The most favorable temperature range for indicator bacteria was 27 C, Fecal bacteria were still found in samples hold for 103 days at 4 and 27 C; the Log10 of colony form unit (cfu) per gram of manure sample released into water was ranging from 1.1 to 3.6 for fecal coliforms, 1.0 to 3.2 for E.coli, and 4.9 5.4 for fecal coliforms, and streptococci.