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The ability to estimate plant water content may provide valuable information to environmental and irrigation system managers to relieve dehydration symptoms and prevent permanent growth and production damage. A portable spectroradiometer was used to gather hyperspectral reflectance data from three plant species (corn, spinach and snap beans) grown in a greenhouse and subjected to different watering conditions to instigate different levels of moisture deficiency. Spectral bands at 950-970, 1150-1260, 1450, 1950, and 2250 nm and five indices (WI, NDVI, SIPI, fWBI, and WI/NDVI) were analyzed to determine the best method of nondestructively estimating plant water content. In corn and snap beans, the 1450 nm band data provided the best estimate (r2 = 0.67 and 0.50). In spinach, reflectance data from the 950-970 nm bands were the most useful (r2 = 0.94).