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In 2001 in Tala, Jalisco (Mexico), a gasoline spill occurred when a gasoline pipeline ruptured. Soil and water body in that area were polluted with gasoline. Raw and Composted Sewage Sludge, and Cattle Manure were incorporated into the soil in order to bioremediate it. Population kinetics of bacteria, filamentous fungi, actinomycete, and hydrocarbon degraders were determined during the first eight weeks of these soil biostimulation treatments. Gasoline-polluted soil showed higher bacterial population and lower fungal and actinomycete populations than unpolluted soil. Biostimulation treatments with Sewage Sludge (Raw or Composted) and Cattle Manure increased populations of bacteria, fungi and actinomycete in addition to microbial activity, while microbial gasoline-users as a C source decreased. Biostimulation treatments promoted the reestablishment of the microbial population to normal conditions faster than Control treatment. Sewage sludge contained considerable amounts of organic matter, nutrients and microbial populations. Hence, this material could contribute to restoration of gasoline-polluted soil.