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Influence of High Shear Bioreactor Parameters on Carbohydrate Release from Different Biomasses

Published by the American Society of Agricultural and Biological Engineers, St. Joseph, Michigan

Citation:  2008 Providence, Rhode Island, June 29 – July 2, 2008  084109.(doi:10.13031/2013.24960)
Keywords:   Keywords: Big blue stem, corn stover, enzyme hydrolysis, extrusion, pretreatment, screw speed, switchgrass, temperature

Abstract: World is moving towards the development of alternative fuels due to many obvious reasons. Biomass will be the main feedstock because of its abundance, renewable nature and cost. Unlike grain starch, biomass carbohydrates are not readily accessible to enzymatic digestion, and hence pretreatment becomes inevitable. A number of different pretreatments involving biological, chemical, physical, and thermal approaches have been investigated over the years with pros and cons. Extrusion is a well established process in food industries; it can be used as a physical pretreatment method for biomass. With the objective of evaluating the effect of high shear parameters on carbohydrate release from different biomasses, corn stover, switchgrass, and big blue stem were pretreated in high shear bioreactor by varying the barrel temperature and screw speed from 50 to 200°C and 50 to 200 rpm, respectively. Enzymatic hydrolysis of pretreated samples was carried out using multi-enzyme complex and ß glucosidase. Screw speed and temperature had significant effect on different sugars conversion from these biomasses. The highest glucose and combined sugar conversion of 22.76 and 43.33, 41.17 and 68.33, 26.44 and 43.63% were recorded at 50°C and 150 rpm for corn stover, at 50°C and 100 rpm and at 100°C and 150 for switchgrass and at 150°C and 200 rpm for big blue stem, respectively. The increase in combined sugar conversion was 12, 23 and 76% for corn stover, big blue stem and switchgrass, respectively over the control samples.

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